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dc.contributor.authorSoininen, Eeva M
dc.contributor.authorValentini, Alice
dc.contributor.authorCoissac, Eric
dc.contributor.authorMiquel, Christian
dc.contributor.authorGielly, Ludovic
dc.contributor.authorBrochmann, Christian
dc.contributor.authorBrysting, Anne K
dc.contributor.authorSønstebø, Jørn H
dc.contributor.authorIms, Rolf A
dc.contributor.authorYoccoz, Nigel G
dc.contributor.authorTaberlet, Pierre
dc.date.accessioned2015-10-09T02:12:00Z
dc.date.available2015-10-09T02:12:00Z
dc.date.issued2009
dc.identifier.citationFrontiers in Zoology. 2009 Aug 20;6(1):16
dc.identifier.urihttp://hdl.handle.net/10852/46728
dc.description.abstractBackground In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus, with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trnL (UAA) intron. Results Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores.
dc.language.isoeng
dc.rightsSoininen et al; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleAnalysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
dc.typeJournal article
dc.date.updated2015-10-09T02:12:01Z
dc.creator.authorSoininen, Eeva M
dc.creator.authorValentini, Alice
dc.creator.authorCoissac, Eric
dc.creator.authorMiquel, Christian
dc.creator.authorGielly, Ludovic
dc.creator.authorBrochmann, Christian
dc.creator.authorBrysting, Anne K
dc.creator.authorSønstebø, Jørn H
dc.creator.authorIms, Rolf A
dc.creator.authorYoccoz, Nigel G
dc.creator.authorTaberlet, Pierre
dc.identifier.doihttp://dx.doi.org/10.1186/1742-9994-6-16
dc.identifier.urnURN:NBN:no-50926
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/46728/1/12983_2009_Article_104.pdf
dc.type.versionPublishedVersion
cristin.articleid16


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