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dc.contributor.authorMinge, Marianne A
dc.contributor.authorShalchian-Tabrizi, Kamran
dc.contributor.authorTørresen, Ole K
dc.contributor.authorTakishita, Kiyotaka
dc.contributor.authorProbert, Ian
dc.contributor.authorInagaki, Yuji
dc.contributor.authorKlaveness, Dag
dc.contributor.authorJakobsen, Kjetill S
dc.date.accessioned2015-10-09T02:11:37Z
dc.date.available2015-10-09T02:11:37Z
dc.date.issued2010
dc.identifier.citationBMC Evolutionary Biology. 2010 Jun 21;10(1):191
dc.identifier.urihttp://hdl.handle.net/10852/46712
dc.description.abstractBackground Plastid replacements through secondary endosymbioses include massive transfer of genes from the endosymbiont to the host nucleus and require a new targeting system to enable transport of the plastid-targeted proteins across 3-4 plastid membranes. The dinoflagellates are the only eukaryotic lineage that has been shown to have undergone several plastid replacement events, and this group is thus highly relevant for studying the processes involved in plastid evolution. In this study, we analyzed the phylogenetic origin and N-terminal extensions of plastid-targeted proteins from Lepidodinium chlorophorum, a member of the only dinoflagellate genus that harbors a green secondary plastid rather than the red algal-derived, peridinin-containing plastid usually found in photosynthetic dinoflagellates. Results We sequenced 4,746 randomly picked clones from a L. chlorophorum cDNA library. 22 of the assembled genes were identified as genes encoding proteins functioning in plastids. Some of these were of green algal origin. This confirms that genes have been transferred from the plastid to the host nucleus of L. chlorophorum and indicates that the plastid is fully integrated as an organelle in the host. Other nuclear-encoded plastid-targeted protein genes, however, are clearly not of green algal origin, but have been derived from a number of different algal groups, including dinoflagellates, streptophytes, heterokonts, and red algae. The characteristics of N-terminal plastid-targeting peptides of all of these genes are substantially different from those found in peridinin-containing dinoflagellates and green algae. Conclusions L. chlorophorum expresses plastid-targeted proteins with a range of different origins, which probably arose through endosymbiotic gene transfer (EGT) and horizontal gene transfer (HGT). The N-terminal extension of the genes is different from the extensions found in green alga and other dinoflagellates (peridinin- and haptophyte plastids). These modifications have likely enabled the mosaic proteome of L. chlorophorum.
dc.language.isoeng
dc.rightsMinge et al; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleA phylogenetic mosaic plastid proteome and unusual plastid-targeting signals in the green-colored dinoflagellate Lepidodinium chlorophorum
dc.typeJournal article
dc.date.updated2015-10-09T02:11:38Z
dc.creator.authorMinge, Marianne A
dc.creator.authorShalchian-Tabrizi, Kamran
dc.creator.authorTørresen, Ole K
dc.creator.authorTakishita, Kiyotaka
dc.creator.authorProbert, Ian
dc.creator.authorInagaki, Yuji
dc.creator.authorKlaveness, Dag
dc.creator.authorJakobsen, Kjetill S
dc.identifier.cristin347274
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2148-10-191
dc.identifier.urnURN:NBN:no-50908
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/46712/1/12862_2009_Article_1407.pdf
dc.type.versionPublishedVersion
cristin.articleid191


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