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dc.contributor.authorTibballs, Katrina L
dc.contributor.authorHerman Ambur, Ole
dc.contributor.authorAlfsnes, Kristian
dc.contributor.authorHomberset, Håvard
dc.contributor.authorFrye, Stephan A
dc.contributor.authorDavidsen, Tonje
dc.contributor.authorTønjum, Tone
dc.date.accessioned2015-10-09T02:10:29Z
dc.date.available2015-10-09T02:10:29Z
dc.date.issued2009
dc.identifier.citationBMC Microbiology. 2009 Jan 09;9(1):7
dc.identifier.urihttp://hdl.handle.net/10852/46664
dc.description.abstractBackground Neisseria meningitidis, the causative agent of meningococcal disease, is exposed to high levels of reactive oxygen species inside its exclusive human host. The DNA glycosylase Fpg of the base excision repair pathway (BER) is a central player in the correction of oxidative DNA damage. This study aimed at characterizing the meningococcal Fpg and its role in DNA repair. Results The deduced N. meningitidis Fpg amino acid sequence was highly homologous to other Fpg orthologues, with particularly high conservation of functional domains. As for most N. meningitidis DNA repair genes, the fpg gene contained a DNA uptake sequence mediating efficient transformation of DNA. The recombinant N. meningitidis Fpg protein was over-expressed, purified to homogeneity and assessed for enzymatic activity. N. meningitidis Fpg was found to remove 2,6-diamino-4-hydroxy-5-formamidopyrimidine (faPy) lesions and 7,8-dihydro-8-oxo-2'-deoxyguanosine (8oxoG) opposite of C, T and G and to a lesser extent opposite of A. Moreover, the N. meningitidis fpg single mutant was only slightly affected in terms of an increase in the frequency of phase variation as compared to a mismatch repair mutant. Conclusion Collectively, these findings show that meningococcal Fpg functions are similar to those of prototype Fpg orthologues in other bacterial species.
dc.language.isoeng
dc.rightsTibballs et al.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleCharacterization of the meningococcal DNA glycosylase Fpg involved in base excision repair
dc.typeJournal article
dc.date.updated2015-10-09T02:10:30Z
dc.creator.authorTibballs, Katrina L
dc.creator.authorHerman Ambur, Ole
dc.creator.authorAlfsnes, Kristian
dc.creator.authorHomberset, Håvard
dc.creator.authorFrye, Stephan A
dc.creator.authorDavidsen, Tonje
dc.creator.authorTønjum, Tone
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2180-9-7
dc.identifier.urnURN:NBN:no-50820
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/46664/1/12866_2008_Article_678.pdf
dc.type.versionPublishedVersion
cristin.articleid7


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