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dc.contributor.authorKorsmo, Heidi Anine
dc.date.accessioned2014-12-08T23:00:11Z
dc.date.issued2014
dc.identifier.citationKorsmo, Heidi Anine. Immune Cell Composition in Human Non-Small Cell Lung Cancer. Master thesis, University of Oslo, 2014
dc.identifier.urihttp://hdl.handle.net/10852/41710
dc.description.abstractBackground: Tumor-infiltrating immune cells analyzed by immunohistochemistry are reported as alternative prognostic factors to supplement TNM staging in operable non-small cell lung cancer (NSCLC), but little is done by flow cytometry. Method: We established a protocol to analyze immune cells in tumor, distant lung (far from tumor) and peripheral blood mononuclear cells (PBMCs) by 8 color flow cytometry. 30 NSCLC patients with adenocarcinoma, squamous cell carcinoma and more uncommon histotypes were included and statistical analyses of cell subsets were performed. Results: We have identified the following tumor-infiltrating immune cells: CD45+ leukocytes, granulocytes, CD19+ B cells, CD4+ and CD8+ T cells with naïve/memory phenotype, CD56+CD16+ and CD56+CD16- NK cells, CD14+ macrophages, CD123+ plasmacytoid dendritic cells (pDCs), CD1c+ myeloid dendritic cells (mDCs) and CD141+ mDCs. There was an increase in leukocyte numbers in tumor compared with distant lung in adenocarcinoma. When expressed as percent of total leukocytes, we found an increase in B cells in tumor compared with distant lung in both adenocarcinoma and squamous cell carcinoma. Squamous cell carcinoma tumors had a decrease of CD141+ mDCs compared with distant lung. Tumor-infiltrating immune cells between histotypes did not reveal differences in composition. TNM stages did not affect tumor-infiltrating immune cell composition in adenocarcinoma. Smoking history showed a decrease in CD141+ mDCs in present compared with previous smokers. PD-1 staining indicates strong variation between patients. Conclusions: Tumor microenvironment recruits immune cells and have a different composition than distant lung. Different composition of tumor-infiltrating immune cells between patients may be of future prognostic value.eng
dc.language.isoeng
dc.subjectTumorimmunology
dc.subjectnon
dc.subjectsmall
dc.subjectcell
dc.subjectlung
dc.subjectcancer
dc.subjectNSCLC
dc.subjectimmune
dc.subjectcell
dc.subjectcomposition
dc.subjectFlow
dc.subjectcytometry
dc.titleImmune Cell Composition in Human Non-Small Cell Lung Cancereng
dc.typeMaster thesis
dc.date.updated2014-12-08T23:00:11Z
dc.creator.authorKorsmo, Heidi Anine
dc.date.embargoenddate3014-08-31
dc.rights.termsDette dokumentet er ikke elektronisk tilgjengelig etter ønske fra forfatter. Tilgangskode/Access code A
dc.identifier.urnURN:NBN:no-46170
dc.type.documentMasteroppgave
dc.rights.accessrightsclosedaccess
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/41710/7/2014-Korsmo-Master-Thesis.pdf


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