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dc.date.accessioned2013-12-19T15:27:22Z
dc.date.available2013-12-19T15:27:22Z
dc.date.created2013-12-18T10:24:40Z
dc.date.issued2013
dc.identifier.citationBooth, James A. Thomassen, Gard O Sundby Rowe, Alexander D. Weel-Sneve, Ragnhild Lagesen, Karin Kristiansen, Knut Ivan Bjørås, Magnar Rognes, Torbjørn Lindvall, Jessica Margareta . Tiling array study of MNNG treated Escherichia coli reveals a widespread transcriptional response. Scientific Reports. 2013, 3
dc.identifier.urihttp://hdl.handle.net/10852/37932
dc.description.abstractThe alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon – consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli following a MNNG challenge. Along with the expected upregulation of the adaptive response genes (ada, alkA and alkB), we identified a number of differentially expressed transcripts, both novel and annotated. This indicates a wider regulatory response than previously documented. There were 250 differentially-expressed and 2275 similarly-expressed unannotated transcripts. We found novel upregulation of several stress-induced transcripts, including the SOS inducible genes recN and tisAB, indicating a novel role for these genes in alkylation repair. Furthermore, the ada-regulon A and B boxes were found to be insufficient to explain the regulation of the adaptive response genes after MNNG exposure, suggesting that additional regulatory elements must be involved.
dc.languageEN
dc.rightsAttribution 3.0 Unported
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleTiling array study of MNNG treated Escherichia coli reveals a widespread transcriptional response
dc.typeJournal article
dc.creator.authorBooth, James A.
dc.creator.authorThomassen, Gard O Sundby
dc.creator.authorRowe, Alexander D.
dc.creator.authorWeel-Sneve, Ragnhild
dc.creator.authorLagesen, Karin
dc.creator.authorKristiansen, Knut Ivan
dc.creator.authorBjørås, Magnar
dc.creator.authorRognes, Torbjørn
dc.creator.authorLindvall, Jessica Margareta
cristin.unitcode185,53,18,11
cristin.unitnameAvdeling for mikrobiologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1078396
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=3&rft.spage=&rft.date=2013
dc.identifier.jtitleScientific Reports
dc.identifier.volume3
dc.identifier.pagecount10
dc.identifier.doihttp://dx.doi.org/10.1038/srep03053
dc.identifier.urnURN:NBN:no-40029
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn2045-2322
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/37932/2/srep03053.pdf
dc.type.versionPublishedVersion
cristin.articleid3053


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