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(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
Abstract
Immunocompromised patients often fail to raise protective vaccine-induced immunity against the global emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Although monoclonal ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Abstract
TCR-like antibodies represent a unique type of engineered antibodies with specificity toward pHLA, a ligand normally restricted to the sensitive recognition by T cells. Here, we report a phage display-based ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Introduction
The acquisition of humoral immunity in utero is essential for the fetus. The crucial protein, which is responsible for this part of immunity, is immunoglobulin-G (IgG). Immune functions of IgGs are mediated ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Monoclonal IgG antibodies are the fastest growing class of biologics, but large differences exist in their plasma half-life in humans. Thus, to design IgG antibodies with favorable pharmacokinetics, it is crucial to identify ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Abstract Antibody-based therapeutics (ABTs) are used to treat a range of diseases. Most ABTs are either full-length IgG1 antibodies or fusions between for instance antigen (Ag)-binding receptor domains and the IgG1 Fc ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
Targeted delivery of antigen to antigen-presenting cells (APCs) enhances antigen presentation and thus, is a potent strategy for making more efficacious vaccines. This can be achieved by use of antibodies with specificity ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
Albumin has a serum half-life of 3 weeks in humans. This feature can be used to improve the pharmacokinetics of shorter-lived biologics. For instance, an albumin-binding domain (ABD) can be used to recruit albumin. A ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2021)
Antibodies specific for peptides bound to human leukocyte antigen (HLA) molecules are valuable tools for studies of antigen presentation and may have therapeutic potential. Here, we generated human T cell receptor (TCR)–like ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2020)
Needle-free uptake across mucosal barriers is a preferred route for delivery of biologics, but the efficiency of unassisted transmucosal transport is poor. To make administration and therapy efficient and convenient, ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
Albumin has an average plasma half-life of three weeks and is thus an attractive carrier to improve the pharmacokinetics of fused therapeutics. The half-life is regulated by FcRn, a cellular receptor that protects against ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
Tripartite motif containing-21 (TRIM21) is a cytosolic ubiquitin ligase and antibody receptor that provides a last line of defense against invading viruses. It does so by acting as a sensor that intercepts antibody-coated ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
The complement system is vital for anti-microbial defense. In the classical pathway, pathogen-bound antibody recruits the C1 complex (C1qC1r2C1s2) that initiates a cleavage cascade involving C2, C3, C4, and C5 and triggering ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
Monoclonal antibodies (mAbs) are valuable as research reagents, in diagnosis and in therapy. Their high specificity, the ease in production, favorable biophysical properties and the opportunity to engineer different ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
Antibodies are essential components of an adaptive immune response. Immunoglobulin G (IgG) is the most common type of antibody found in circulation and extracellular fluids. Although IgG alone can directly protect the body ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2019)
Background & Aims
Development of celiac disease is believed to involve the transglutaminase-dependent response of CD4+ T cells toward deamidated gluten peptides in the intestinal mucosa of individuals with specific ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2018)
There is a quest for production of soluble protein of high quality for the study of T-cell receptors (TCRs), but expression often results in low yields of functional molecules. In this study, we used an E. coli chaperone-assisted ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2018)
Albumin and IgG have remarkably long serum half-lives due to pH-dependent FcRn-mediated cellular recycling that rescues both ligands from intracellular degradation. Furthermore, increase in half-lives of IgG and albumin-based ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2018)
Albumin has a long serum half-life due to its unique ability to bind the cellular neonatal Fc receptor (FcRn), which provides protection from intracellular degradation. The interaction can be capitalized to improve the ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2018)
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2017)
The neonatal crystallizable fragment receptor (FcRn) is responsible for maintaining the long half-life and high levels of the two most abundant circulating proteins, albumin and IgG. In the latter case, the protective ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)
Selection of biased T cell receptor (TCR) repertoires across individuals is seen in both infectious diseases and autoimmunity, but the underlying molecular basis leading to these shared repertoires remains unclear. Celiac ...
(Journal article / Tidsskriftartikkel / PublishedVersion, 2017)
This Article contains a typographical error in the Methods section under the subheading ‘Crystallization’, where the Protein Data Bank accession code ‘5LDN’ was incorrectly given as ‘5LDV’.
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)
During infection with non-enveloped viruses, antibodies stimulate immunity from inside cells by activating the cytosolic Fc receptor TRIM21. This intracellular humoral response relies on opsonized viral particles reaching ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)
Phage display screening readily allows for the identification of a multitude of antibody specificities, but to identify optimal lead candidates remains a challenge. Here, we direct the antibody-capsid fusion away from the ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
Eculizumab is a humanized IgG2/4 chimeric anti-complement C5 antibody used to treat patients with paroxysmal nocturnal hemoglobinuria (PNH) or atypical hemolytic uremic syndrome. The aim of this study was to evaluate whether ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
Cancer immunotherapy has finally come of age, demonstrated by recent progress in strategies that engage the endogenous adaptive immune response in tumor killing. Occasionally, significant and durable tumor regression has ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
Antibodies are key molecules in the fight against infections. Although previously thought to mediate protection solely in the extracellular environment, recent research has revealed that antibody-mediated protection extends ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
The remarkable clinical success of Fc-fusion proteins has driven intense investigation for even more potent replacements. Using quality-by-design (QbD) approaches, we generated hexameric-Fc (hexa-Fc), a ~20 nm oligomeric ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
The neonatal Fc receptor (FcRn) was first found to be responsible for transporting antibodies of the immunoglobulin G (IgG) class from the mother to the fetus or neonate as well as for protecting IgG from intracellular ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
A major challenge for the therapeutic use of many peptides and proteins is their short circulatory half-life. Albumin has an extended serum half-life of 3 weeks because of its size and FcRn-mediated recycling that prevents ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
Albumin is an abundant blood protein that acts as a transporter of a plethora of small molecules like fatty acids, hormones, toxins, and drugs. In addition, it has an unusual long serum half-life in humans of nearly 3 ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
Albumin is the most abundant protein in blood and plays a pivotal role as a multitransporter of a wide range of molecules such as fatty acids, metabolites, hormones, and toxins. In addition, it binds a variety of drugs. ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2013)
FcRn is a key player in several immunological and non-immunological processes, as it mediates maternal-fetal transfer of IgG, regulates the serum persistence of IgG and albumin, and transports both ligands between different ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2013)
Albumin has a serum half-life of 3 weeks in humans. This has been utilized to extend the serum persistence of biopharmaceuticals that are fused to albumin. In light of the fact that the neonatal Fc receptor (FcRn) is a key ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2013)
We here report a novel phage display selection strategy enabling fast and easy selection of thermostabilized proteins. The approach is illustrated with stabilization of an aggregation-prone soluble single chain T cell ...