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dc.date.accessioned2013-03-12T13:33:20Z
dc.date.issued2007en_US
dc.date.submitted2007-11-29en_US
dc.identifier.citationBreivik, Vera, , Nguyen, Nguyen Nguyen, , Pervaiz, Maryam, , . Bacteria in apical and marginal periodontitis detected with DNA-DNA hybridization technique. Prosjektoppgave, University of Oslo, 2007en_US
dc.identifier.urihttp://hdl.handle.net/10852/33138
dc.description.abstractAbstract: In the present study the Checkerboard DNA-DNA hybridization technique was used to identify bacteria in persistent periapical lesions and recurrent marginal periodontitis. A second aim of the study was to investigate if two different flap designs during surgery would influence the detection rate of bacterial DNA in periradicular tissue. The study included 68 patients. 24 patients had the diagnosis recurrent marginal periodontitis (group A), and 44 patients had root-filled teeth with persistent apical periodontitis (Group B). The group with apical periodontitis was divided into two subgroups, B1, and B2. In B1, which included 23 patients, a marginal incision was performed during surgery, and in B2, including 21 patients, a submarginal incision was applied. Bacterial DNA was identified in all samples from the three groups using 20 different whole genomic probes. The mean number of species detected was 13. 3 in Group A, 11. 4 in group B1 and 9. 7 in group B2. Porphyromonas gingivalis, Prevotella nigrescens, Treponema denticola, Actinomyces israelii, Actinomyses viscosus, Peptostreptococcus micros, E ubacterium saburreum and Treponema socranskii dominated in all three groups. P. gingivalis was present in 83% of the lesions in group A, 78% in group B1 and in all lesions in B2. T. denticola was present in 95% in group A and in all lesions in group B1 and B2. The third bacteria in the red complex , T. forsythia was present in 79% in group A, in 52% of the lesions in group B1 and 39% in group B2. Species present in high numbers (> 106 cells) in group A (in more than three lesions) were: P. gingivalis, A. actinomycetemcomitans, T. denticola, P. nigrescens, F. nucleatum ssp polymorphum, T. socranskii ssp. socranskii, Peptostreptococcus micros, S. intermedius. E. saburreum, T. denticola. P. nigrescens were present in high numbers in group B1 and T. denticola and P. nigrescens in group B2. A consortium of different species, both anaerobic and facultative anaerobic species, were identified in recurrent marginal periodontitis and in persistent periapical lesions.nor
dc.language.isoengen_US
dc.titleBacteria in apical and marginal periodontitis detected with DNA-DNA hybridization techniqueen_US
dc.typeMaster thesisen_US
dc.date.updated2010-01-29en_US
dc.creator.authorBreivik, Veraen_US
dc.creator.authorNguyen, Nguyen Nguyenen_US
dc.creator.authorPervaiz, Maryamen_US
dc.date.embargoenddate10000-01-01
dc.rights.termsKLAUSULERING: Dokumentet er klausulert grunnet lovpålagt taushetsplikt. Tilgangskode/Access code Cen_US
dc.rights.termsforeveren_US
dc.subject.nsiVDP::710en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Breivik, Vera&rft.au=Nguyen, Nguyen Nguyen&rft.au=Pervaiz, Maryam&rft.title=Bacteria in apical and marginal periodontitis detected with DNA-DNA hybridization technique&rft.inst=University of Oslo&rft.date=2007&rft.degree=Prosjektoppgaveen_US
dc.identifier.urnURN:NBN:no-21820en_US
dc.type.documentProsjektoppgaveen_US
dc.identifier.duo68377en_US
dc.contributor.supervisorPia Titterud Sundeen_US
dc.identifier.bibsys092120601en_US
dc.rights.accessrightsclosedaccessen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/33138/1/thesis.pdf


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