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Now showing items 1-10 of 23
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2017)
Immunocapture-based bottom-up LC–MS is a promising technique for the quantification of low abundant proteins. Magnetic immunocapture beads provide efficient enrichment from complex samples through the highly specific ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2017)
Molecularly imprinted polymers (MIPs) have been used as useful sorbents in solid-phase extraction for a wide range of molecules and sample matrices. Their unique selectivity can be fine-tuned in the imprinting process and ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2017)
A concept integrating sampling and protein digestion is introduced here combining fast and simple fabrication by wax printing on filter paper with trypsin immobilized polymer beads. The paper reactors showed promising ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)
Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2017)
Determination of proteins in complex biological matrices has massive attention and is exploited in many different scientific disciplines. Routinely, proteins are determined using antibody based immuno-metric assays. Although ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)
The use of antibodies in “bottom-up “LC-MS workflows to determine low abundant biological active proteins in complex human samples has increased in recent years: immuno-capture analysis combines the workflow of conventional ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2018)
Using LC-MS/MS for determination of low-abundance protein biomarkers from dried blood spots is challenging due to the combination of low biomarker levels (low pM-level) and small sample volumes (typically <50 μL). In the ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2018)
The aim was to evaluate the performance of different microsampling materials in LC–MS-based protein analysis. The evaluated materials were the Volumetric Absorptive MicroSampling (VAMS) device, the pure cellulose sampling ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
This paper describes smart sampling paper to be used for bottom-up protein analysis. Four different manners to immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized paper (with ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
Antibody-based affinity capture has become the gold standard in sample preparation for determination of low-abundance protein biomarkers in biological matrices prior to liquid chromatography-mass spectrometry (LC-MS) ...