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dc.date.accessioned2013-03-12T12:48:39Z
dc.date.available2013-03-12T12:48:39Z
dc.date.issued2007en_US
dc.date.submitted2007-11-27en_US
dc.identifier.citationPham, Trung. Distribution of Mycobacterium tuberculosis lineages overview in the North of Vietnam. Masteroppgave, University of Oslo, 2007en_US
dc.identifier.urihttp://hdl.handle.net/10852/30146
dc.description.abstractABSTRACT Distribution of Mycobacterium tuberculosis lineages, Overview in Northern - Vietnam Author: Pham Trung M.D. Supervisors: Gunnar Bjune, Prof., M.D., Ph.D.; Ulf R. Dahle, Senior scientist Co-supervisors: Vu Tan Trao, Ass. Prof., M.D., Ph.D.; Bui Duc Duong, M.D., Ph.D. Sponsor: University of Oslo, Faculty of Medicine, Institute of General Practice and Community Medicine, Section for International Health. Vietnam was among the high-burden tuberculosis countries. Molecular typing is not available on a routine basis in the Vietnamese National Tuberculosis Program’s laboratories. DNA fingerprinting of Mycobacterium tuberculosis is an international standard epidemiologic tool, which exploits variability in both the number and genomic position of Insertion Sequence 6110. This method allows generating strain specific DNA patterns (DNA fingerprints). Besides, IS6110-Restriction Fragment Length Polymorphism (RFLP) analysis has proven useful for the recognition of outbreaks in communities, modes transmission and determination of genetic diversity in M. tuberculosis populations world-wide. To understand “Emerging strains” of M. tuberculosis and their epidemiology and distribution, RFLP studies are considered valuable for the public health aspects of the Vietnamese National Tuberculosis Program. Sputum smears collected, AFB microscopy performed and M. tuberculosis strains were grown on Löwenstein-Jensen slants for 4 to 6 weeks at local hospitals. PCR was used to detect M. tuberculosis DNA and a probe hybridization technique was used for genotyping. DNA was extracted, digested and separated by using horizontal 0.8% agarose gels in Tris-acetate buffer and vacuum blotted onto nylon membranes. For the IS6110 RFLP, the DNA was hybridized to the 254-bp PCR product of IS6110 and visualized by a digoxigenin-dUTP labelling and detection kit (Roche Diagnostics GmbH, Mannheim, Germany) in Norwegian Institute of Public Health (NIPH) in Norway. The sample size of this study was too small to make conclusive recommendations. However, clear indications arise. The East African Indian family appears to represent a well established epidemic and presented an epidemiological pattern indicating recent transmission both within and between urban and rural areas. The Beijing and other families however, appeared to represent isolates of ongoing importation to northern of Vietnam. Multi-drug resistance was not found to be related to any particular Mycobacterium tuberculosis family. Despite the presence of several M. tuberculosis lineages and the large genetic diversity in the MTB population presented in northern Vietnam, spoligotyping should be considered valuable screening method for clustering of M. tuberculosis isolates and their assignment to known genotypes. Also the affordability reproducibility of the method is in its advantagenor
dc.language.isoengen_US
dc.titleDistribution of Mycobacterium tuberculosis lineages overview in the North of Vietnamen_US
dc.typeMaster thesisen_US
dc.date.updated2008-01-18en_US
dc.creator.authorPham, Trungen_US
dc.subject.nsiVDP::751en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Pham, Trung&rft.title=Distribution of Mycobacterium tuberculosis lineages overview in the North of Vietnam&rft.inst=University of Oslo&rft.date=2007&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-17888en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo68297en_US
dc.contributor.supervisorGunnar Bjune, Ulf R. Dahle, Vu Tan Trao, Bui Duc Duongen_US
dc.identifier.bibsys080083366en_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/30146/2/PhamxTrung.pdf


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