Aim: The present study was designed to elucidate in detail the role of stroke volume (SV), heart rate (HR) and total peripheral resistance (TPR) in the pressor response during isometric exercise. Previous published studies have shown different results considering the role of SV. Different protocols may be one possible explanation for the discrepancy between the results. The main difference between the protocols is the use of a cuff inflated to suprasystolic pressure applied on the upper part of the working arm. We have therefore studied the effect of isometric exercise both with, and without applying such a cuff during the exercise. Methods: In fourteen volunteers arterial blood pressure, beat-to-beat SV, HR, acral and non-acral skin perfusion were continuously measured before, during, and after periods of two minutes isometric handgrip of 40 % maximal voluntary contraction force. Cardiac output and TPR were then calculated. Isometric handgrip was performed both with and without additional application of an inflated cuff, in to different protocols, both done by each of the participants. Results: In both protocols MAP, HR and TPR rose, while stroke volume decreased during isometric exercise. Conclusions: SV did not contribute to the pressor response in isometric exercise. The same pattern was observed when occlusion of the bloodstream to the working arm was applied, witch means that the two different protocols can not explain the different results in previous published studies. An increase in HR is the most important factor behind the observed increase in MAP during isometric exercise.