• English
    • Norsk
  • English 
    • English
    • Norsk
  • Administration
View Item 
  •   Home
  • Det medisinske fakultet
  • Profesjonsstudiet medisin
  • Ernæringslære
  • View Item
  •   Home
  • Det medisinske fakultet
  • Profesjonsstudiet medisin
  • Ernæringslære
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates.

Avellan, Cecilia I. A.
Master thesis
View/Open
Prosjektxavellan.pdf (1.288Mb)
Year
2008
Permanent link
http://urn.nb.no/URN:NBN:no-20707

Metadata
Show metadata
Appears in the following Collection
  • Ernæringslære [23]
Abstract
Cyclic AMP-dependent protein kinase (PKA) is a holoenzyme composed of two catalytic (C) subunits and a regulatory (R) subunit dimer. The holoenzyme dissociates when four cAMP molecules bind to the R subunit dimer. This activates the C subunits, allowing them to phosphorylate specific substrate proteins and thereby alter their activity. Studies involving cDNA cloning have revealed four different R and four different C subunit genes which encode the subunits designated RI, RI, RII, RII, C, C, C and PRKX1. Furthermore, different splice variants encoded by the human C and C genes have been identified and designated Cα1, CαS, Cβ1, Cβ2, C3, Cβ3b, Cβ3ab, Cβ3abc, C4, Cβ4b, C4ab and C4abc. They are all tissue-specifically expressed, highly heterogenic in the N-terminal end, and highly conserved in the catalytic domain.

We have discovered 6 novel splice variants of the C gene in NTera2-N cells as both mRNAs and proteins. All the novel splice variants were designated CβΔ4 since they lack 99 encoding base pairs representing exon 4 due to inframe skipping in the C gene. We developed a method to detect CβΔ4 mRNAs and found that these are primarily expressed in the brain of higher primates. While separately co-expressing different CβΔ4 variants with either the RI or RII subunit, we demonstrated that CβΔ4 interacts with the R subunit, in a cAMP insensitive fashion. This together with the fact that these variants are brain specifically expressed may suggest that they function to regulate cAMP sensitivity of nerve cells.
 
Responsible for this website 
University of Oslo Library


Contact Us 
duo-hjelp@ub.uio.no


Privacy policy
 

 

For students / employeesSubmit master thesisAccess to restricted material

Browse

All of DUOCommunities & CollectionsBy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

For library staff

Login
RSS Feeds
 
Responsible for this website 
University of Oslo Library


Contact Us 
duo-hjelp@ub.uio.no


Privacy policy