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dc.date.accessioned2013-03-12T12:25:08Z
dc.date.available2013-03-12T12:25:08Z
dc.date.issued2007en_US
dc.date.submitted2007-08-03en_US
dc.identifier.citationRæder, Hanna. Transcriptional regulation of the LSDP5 gene. Masteroppgave, University of Oslo, 2007en_US
dc.identifier.urihttp://hdl.handle.net/10852/28673
dc.description.abstractLipid droplet storage proteins (LSDPs) associate with lipid droplets and are involved in the molecular processes of deposition and catabolism of stored lipids in the cells. These proteins differ in tissue distribution, binding affinity to lipid storage droplets, protein stability and transcriptional regulation. Due to these differences, they are believed to have evolved to fine tune fatty acid metabolism according to the particular needs of various tissues. Recent knowledge reveals that these proteins are transcriptionally regulated by Peroxisome Proliferator-Activated Receptors (PPAR). In this thesis I have focused on the newly discovered protein, lipid storage droplet protein 5 (LSDP5), and the transcriptional regulation of this protein. Nebbs group has previously demonstrated in animal studies that LSDP5 is transcriptionally regulated by PPARá in liver. However, it has been difficult to prove through transfection studies that this regulation is mediated through a PPARá responsive DR1 element (PPRE). The main objective for this thesis was thus to establish whether there is a functional PPRE located in the LSDP5 promoter. Furthermore, the aims were to examine whether the transcriptional regulation of LSDP5 is affected by additional regulatory factors; the transcription factor estrogen-related receptor á (ERRá) and the coactivator PPARã coactivator-1á (PGC-1á). In this study it was confirmed that LSDP5 contains a functional PPRE, located in the -2077base pairs (bp) to -2064bp upstream region in the LSDP5 gene. Furthermore, it was demonstrated that the PPARá mediated transcriptional regulation of LSDP5 is dependent on PGC-1á. PGC-1á enhanced the transcriptional activity in the LSDP5 gene remarkably. ERRá repressed the expression of LSDP5. We observed that the presence of ERRá reduced the enhancing effect of PGC-1á on LSDP5 induction. Taken together, my data and the previously published data in our group confirm that LSDP5 is a PPARá target gene. Furthermore, our findings provide new aspects in the regulation of LSDP5, regarding the role PGC-1á and ERRá.nor
dc.language.isoengen_US
dc.titleTranscriptional regulation of the LSDP5 geneen_US
dc.typeMaster thesisen_US
dc.date.updated2007-11-13en_US
dc.creator.authorRæder, Hannaen_US
dc.subject.nsiVDP::710en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Ræder, Hanna&rft.title=Transcriptional regulation of the LSDP5 gene&rft.inst=University of Oslo&rft.date=2007&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-17002en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo63910en_US
dc.contributor.supervisorHilde Irene Nebben_US
dc.identifier.bibsys071649883en_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/28673/1/EndeligUtkastMasteroppgaven_240707.pdf


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