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dc.date.accessioned2013-03-12T09:10:15Z
dc.date.available2013-03-12T09:10:15Z
dc.date.issued2007en_US
dc.date.submitted2007-03-14en_US
dc.identifier.citationJankowski, Mikolaj Jan. Determination of peptides in microdialysates using 2-dimensional capillary liquid chromatography coupled to electrospray time-of-flight mass spectrometry (2D-capLC-ESI-TOF-MS). Masteroppgave, University of Oslo, 2007en_US
dc.identifier.urihttp://hdl.handle.net/10852/12803
dc.description.abstractIn the present work, a 2D-capLC-ESI-TOF-MS method for fractionation and separation of peptides in microdialysates is demonstrated. Both comprehensive and quantitative analysis was conducted. The microdialysates samples (335 μL) were loaded onto a 1 mm I.D. x 5 mm loading column packed with 5 μm Kromasil C18 particles by a carrier solution of 0.1 % formic acid in ACN/H2O (5/95, v/v) at a flow rate of 100 μL/min. Back-flushing onto a fractionating 0.32 mm I.D. x 150 mm ZIC-HILIC column packed with 5 μm particles was performed using a decreasing linear solvent ACN/H2O gradient containing 10 mM ammonium acetate at pH 6.8. Dilution of the effluent was done using a solution containing 0.1 % formic acid prior to on-line collection of fractions onto multiple 1 mm I.D. x 5 mm Kromasil C18 column packed with 5 μm particles. Back-flushed elution of the fractions onto a 0.3 mm x 150 mm PLRP-S column packed with 3 μm particles was performed using an increasing stepped solvent ACN/H2O gradient containing 0.1 % formic acid. Positive ESI was performed in the m/z range of 200-1500. A post-column standard was introduced to MS detection when performing target compound analysis. The estimated concentration limit of detection (cLOD) was 0.15 ng/mL.nor
dc.language.isoengen_US
dc.titleDetermination of peptides in microdialysates using 2-dimensional capillary liquid chromatography coupled to electrospray time-of-flight mass spectrometry (2D-capLC-ESI-TOF-MS)en_US
dc.typeMaster thesisen_US
dc.date.updated2007-04-03en_US
dc.creator.authorJankowski, Mikolaj Janen_US
dc.subject.nsiVDP::440en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Jankowski, Mikolaj Jan&rft.title=Determination of peptides in microdialysates using 2-dimensional capillary liquid chromatography coupled to electrospray time-of-flight mass spectrometry (2D-capLC-ESI-TOF-MS)&rft.inst=University of Oslo&rft.date=2007&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-14643en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo54628en_US
dc.contributor.supervisorElsa Lundanes, Tyge Greibrokk, Steven Ray Wilsonen_US
dc.identifier.bibsys070489300en_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/12803/1/MIKOLAJ_MASTER_DONE.pdf


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