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dc.date.accessioned2013-03-12T09:09:13Z
dc.date.available2013-03-12T09:09:13Z
dc.date.issued2004en_US
dc.date.submitted2004-11-12en_US
dc.identifier.citationStorbråten, Espen. Determination of neuropeptides in rat brain tissue using 2-dimensional capillary liquid chromatography coupled to electrospray ion-trap mass spectrometry (2-D capLC ESI-IT-MSn. Hovedoppgave, University of Oslo, 2004en_US
dc.identifier.urihttp://hdl.handle.net/10852/12757
dc.description.abstractTo monitor chemical changes in brain occurring during stress, several rats were put to death in both stressed and non-stressed situations. According to age it was desirable to see which effects stress constitutes in a rat brain. By using rats as experimental animals, the difference in the level of neurotransmitters was studied and compared in a stressed and non-stressed situation for different aged rats. Sample preparation, including solid phase extraction (SPE) on a CBA (carboxy propyl phase) weak cation exchanger (WCX) and a reversed phase (RP) C18 column, was initially performed on rat brain parts to extract the compounds of interest. The extraction resulted in two fractions, A and B, with hydrophobic- and ionic compounds, respectively. The samples were subsequently analysed using the developed 2D capillary LC method employing a strong cation exchanger (SCX) in the first dimension, and a reversed phase C18 column for focusing and separating the analytes in the second dimension. 50 µL of brain tissue extracts were initially loaded and focused on the SCX column and elution was performed by a buffered salt step gradient ranging from 20 – 500 mM ammonium formate. Subsequently, the fractions were trapped on reversed phase trap columns prior to back flushed elution onto the RP analytical column. The separation was conducted using a linear ACN/H2O gradient where the mobile phase contained 10mM acetic acid. Detection was performed by an electrospray ionization ion trap mass spectrometer (ESI-IT-MSn) in the positive mode. The experiments proved abnormal activity in a rat brain during stress, and some compounds with bigger abundance differences in the stressed and non-stressed occasions were found and identified. The system evaluation revealed some fluctuations in the analyte area. With angiotensin II as a test substance, the area had an RSD (%) value of 16 % while the retention time had a corresponding value of 1 %.nor
dc.language.isoengen_US
dc.titleDetermination of neuropeptides in rat brain tissue using 2-dimensional capillary liquid chromatography coupled to electrospray ion-trap mass spectrometry (2-D capLC ESI-IT-MSnen_US
dc.typeMaster thesisen_US
dc.date.updated2004-11-15en_US
dc.creator.authorStorbråten, Espenen_US
dc.subject.nsiVDP::440en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Storbråten, Espen&rft.title=Determination of neuropeptides in rat brain tissue using 2-dimensional capillary liquid chromatography coupled to electrospray ion-trap mass spectrometry (2-D capLC ESI-IT-MSn&rft.inst=University of Oslo&rft.date=2004&rft.degree=Hovedoppgaveen_US
dc.identifier.urnURN:NBN:no-10014en_US
dc.type.documentHovedoppgaveen_US
dc.identifier.duo22263en_US
dc.contributor.supervisorTyge Greibrokk, Elsa Lundanesen_US
dc.identifier.bibsys042227046en_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/12757/1/ThesisxforxthexdegreexofxCand.xScient.-converted.pdf


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