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dc.date.accessioned2013-03-12T09:09:28Z
dc.date.issued2010en_US
dc.date.submitted2010-06-25en_US
dc.identifier.citationJohnsen, Elin. Hydrophilic interaction chromatographic separation of 8 deoxynucleotide triphosphates (dNTPs) and nucleotide triphosphates (NTPs).. Masteroppgave, University of Oslo, 2010en_US
dc.identifier.urihttp://hdl.handle.net/10852/12694
dc.description.abstractEight deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs): ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP, were separated using two 15 cm, 2.1. mm I.D. ZIC-pHILIC columns coupled in series, using simple LC-UV instrumentation. Better separations were obtained with isocratic elution as compared to gradient elution. The temperature markedly affected the selectivity and could be used to fine tune separation. The analysis time was also affected by temperature, as lower temperatures surprisingly reduced the retention of the nucleotides. dNTP/NTP standards could be separated in 35 minutes using a flow rate of 200 µL/minute. In complex cell culture samples dNTP/NTPs could be selectively separated in 70 minutes using a flow rate of 100 µL/minute.eng
dc.language.isoengen_US
dc.subjectnukleotider HILIC LC-UVen_US
dc.titleHydrophilic interaction chromatographic separation of 8 deoxynucleotide triphosphates (dNTPs) and nucleotide triphosphates (NTPs).en_US
dc.typeMaster thesisen_US
dc.date.updated2010-07-01en_US
dc.creator.authorJohnsen, Elinen_US
dc.date.embargoenddate10000-01-01
dc.rights.termsDette dokumentet er ikke elektronisk tilgjengelig etter ønske fra forfatter. Tilgangskode/Access code Aen_US
dc.rights.termsforeveren_US
dc.subject.nsiVDP::440en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Johnsen, Elin&rft.title=Hydrophilic interaction chromatographic separation of 8 deoxynucleotide triphosphates (dNTPs) and nucleotide triphosphates (NTPs).&rft.inst=University of Oslo&rft.date=2010&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-25068en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo103926en_US
dc.contributor.supervisorElsa Lundanes, Tyge Greibrokk, Steven Wilson, Helle Malerøden_US
dc.identifier.bibsys101235240en_US
dc.rights.accessrightsclosedaccessen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/12694/1/masterelinjohnsen.pdf


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