Over the past 2 decades major advancements in phylogenetics have been made and this in turnhas influenced our understanding of eukaryotic evolution. The use of morphological datacombined with sequence data has been the basis for the recent division of eukaryotes into five supergroups . This hypothesis is yet to be proven, as a general lack of both taxon and genesequences has only led to the resolution of some of the groups. The chromalveolates arepossibly the most contested of these groups, and it remains to be proven if they belong to onemajor lineage or several distinct groups. Chromalveolate resolution has been suggested to bedependent on the sampling of residual lineages. One such phylum, Telonemia, has beenrecently inferred with a two-gene phylogeny, indicating an affinity to two groups belonging tothe chromalveolates, the cryptomonads and haptophytes. This placement however was weaklysupported and a more conclusive phylogeny is vital to the further understanding ofchromalveolates and other eukaryotic supergroup relationships. For this purpose anorthologous multigene phylogeny of 9 nuclear protein coding genes and the small subunit(SSU) rRNA gene from Telonema and a broad sampling of eukaryotic taxa was used forphylogenetic inferences. Five of these Telonema protein-coding sequences were amplified forthe purpose this study; the remainder were generated from previous studies. The resulting 10gene global eukaryotic phylogeny is the largest dataset as yet to include broad sampled taxafrom all five of the hypothesized supergroups. The phylogentic analysis again placedTelonema as a deep eukaryotic lineage, increased gene and taxon sampling inferred strongerphylogenetic affinity to the cryptomonads, haptophytes and a third chromalveolate group ofkatablepharids. The backbone of the global eukaryotic phylogeny however could not resolvethe monophyly of chromalveolates, alternatively placing the group into three separate clades.Otherwise, the result demonstrated strong monophyly for thee other supergroups, theunikonts, Rhizaria and for the first time Excavata. The fifth supergroup of plants onlydemonstrated monophyly with two of its three lineages, here identified to be caused byphylogenetic artifacts. A focus toward the addition of both genes and taxa should beprioritised for our further understanding of the relationships between the eukaryoticsupergroups.