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dc.date.accessioned2013-03-12T08:44:14Z
dc.date.available2013-03-12T08:44:14Z
dc.date.issued2004en_US
dc.date.submitted2004-10-29en_US
dc.identifier.citationKvernstuen, Liv Guro. Individual identification of red deer (Cervus elaphus) based on DNA extractions from faeces.. Hovedoppgave, University of Oslo, 2004en_US
dc.identifier.urihttp://hdl.handle.net/10852/11698
dc.description.abstractRecent advances in molecular genetic techniques have made it possible to genotype DNA extracts from non-invasive samples such as hair, faeces, and urine. Subsequent identification of individuals from such material has allowed estimation of important parameters such as population size, immigration rate, and reproductive contribution from immigrants, even without handling the animals. This thesis presents a pilot study assessing the reliability of using faecal samples for genotyping in red deer. The test was preformed on samples of blood and faeces from 42 farmed animals, using six microsatellites. The reliability of the method was assessed through a multiple-tube approach and by comparing faecal genotypes with the corresponding genotypes obtained from blood. DNA of sufficient quality to be genotyped for all six loci was extracted from 23 faeces samples (54.8 %) by a silica-based method. Allelic drop-out was found in 7.7 % of the amplifications from extracts of faeces. Comparison of blood and feces samples showed that correct consensus genotypes were obtained at all loci and samples already after the three first replicates. Probability of identity were estimated to PI = 6.5 x 10-6 for unrelated individuals and PI = 8.0 x 10-3 for siblings. These results suggest that DNA extracted from red deer faeces is a viable source for obtaining reliable individual genotypes, and that it can be achieved by a limited number of replicates.nor
dc.language.isoengen_US
dc.titleIndividual identification of red deer (Cervus elaphus) based on DNA extractions from faeces.en_US
dc.typeMaster thesisen_US
dc.date.updated2005-01-20en_US
dc.creator.authorKvernstuen, Liv Guroen_US
dc.subject.nsiVDP::480en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Kvernstuen, Liv Guro&rft.title=Individual identification of red deer (Cervus elaphus) based on DNA extractions from faeces.&rft.inst=University of Oslo&rft.date=2004&rft.degree=Hovedoppgaveen_US
dc.identifier.urnURN:NBN:no-10119en_US
dc.type.documentHovedoppgaveen_US
dc.identifier.duo21838en_US
dc.contributor.supervisorNils Chr. Stenseth ,Kjetill S. Jakobsen, Atle Mysterud, Øystein Flagstad, Eli K. Ruenessen_US
dc.identifier.bibsys050110403en_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/11698/1/21838.pdf


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