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dc.date.accessioned2013-03-12T08:40:24Z
dc.date.issued2011en_US
dc.date.submitted2011-08-23en_US
dc.identifier.citationMahmood, Aqsa. The antinociceptive effect of the FAAH inhibitior URB597 is blocked by the opioid receptor antagonist naloxone. Masteroppgave, University of Oslo, 2011en_US
dc.identifier.urihttp://hdl.handle.net/10852/11454
dc.description.abstractPrevious studies indicate that inhibition of fatty acid amide hydrolase (FAAH) activity may result in accumulation of endocannabinoids both in the brain and the spinal cord. Furthermore, it has been suggested that the events following inhibition of FAAH activity interact with opioid signaling important for nociceptive modulation. Therefore, electrophysiological extracellular field potential recordings were performed to investigate if inhibition of FAAH activity affects nociceptive signaling in the spinal cord and to examine a possible interaction between FAAH inhibition and opioid signaling. A single stimulus was applied to the sciatic nerve of anaesthetised rats, and as a measurement of nociceptive response, the C-fibre response was quantified. To inhibit the enzymatic activity of FAAH and the opioid receptors, URB597 (1.0 mg/kg i.v.) and naloxone (0.1 μg/μl i.th.) were administred, respectively. Quantitative real time RT-PCR was used to explore if FAAH inhibition is associated with changes in spinal dorsal horn gene expression of the genes encoding Zif and iNOS. The field potential recordings demonstrated that the C-fibre response was reduced after inhibition of FAAH by URB597, and that this inhibition was reversed by blocking of the opioid receptors by naloxone. The gene expression analyses did not show any significant change in the expression of Zif and iNOS following FAAH inhibition. The present data suggest that inhibition of the FAAH enzyme reduces the spinal nociceptive responses and that the underlying mechanism may involve activation of opioid receptors and/or intracellular pathways.eng
dc.language.isoengen_US
dc.titleThe antinociceptive effect of the FAAH inhibitior URB597 is blocked by the opioid receptor antagonist naloxoneen_US
dc.typeMaster thesisen_US
dc.date.updated2012-06-10en_US
dc.creator.authorMahmood, Aqsaen_US
dc.date.embargoenddate10000-01-01
dc.rights.termsDette dokumentet er ikke elektronisk tilgjengelig etter ønske fra forfatter. Tilgangskode/Access code Aen_US
dc.rights.termsforeveren_US
dc.subject.nsiVDP::473en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Mahmood, Aqsa&rft.title=The antinociceptive effect of the FAAH inhibitior URB597 is blocked by the opioid receptor antagonist naloxone&rft.inst=University of Oslo&rft.date=2011&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-29073en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo134215en_US
dc.contributor.supervisorLinda M. Pedersen, Johannes Gjerstaden_US
dc.identifier.bibsys113876777en_US
dc.rights.accessrightsclosedaccessen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/11454/1/Mahmood-masteroppgave.pdf


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