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dc.date.accessioned2013-03-12T08:37:43Z
dc.date.issued2009en_US
dc.date.submitted2009-01-07en_US
dc.identifier.citationAndersland, Kristin, Johansen, Guro Five, . Det antimikrobielle peptidet Plantaricin A permeabiliserer lever- og nyreceller. Masteroppgave, University of Oslo, 2009en_US
dc.identifier.urihttp://hdl.handle.net/10852/11417
dc.description.abstractAntimicrobial peptides are produced by nearly all organisms. Certain antimicrobial peptides from multicellular animals also kill a variety of tumour cells at concentrations not affecting normal eukaryotic cells. Recently, it was reported that also Plantaricin A (PlnA), which is a peptide with antibacterial activity produced by Lactobacillus plantarum, may kill eucaryotic cells. It was shown that PlnA permeabilizes cancerous rat pituitary cells (GH4 cells), whereas normal rat anterior pituitary cells are resistant to the peptide. The same study also showed that exposure of PlnA to the inside of the membrane, did not lead to permeabilization. This thesis involved three tasks. The first was to examine whether PlnA differentiates between various normal cells, and the second task was to elucidate if the membrane permeabilizing effect of PlnA is restricted to cancerous cells. An examination of the permeabilizing effect of PlnA on the outer and inner membrane leaflets was the final task. In order to examine these questions, we have studied primary cultures of rat liver cells (hepatocytes, endothelial- and Kupffer cells), primary culture of rat kidney cells and two epithelial cell lines of primate kidney origin (Vero cells from green monkey and human CAKI-2 cells). The Vero cell line is derived from normal cells, whereas the CAKI-2 cell line is derived from a cancerous tumour. The membrane effects were studied by means of patch clamp recordings and microfluorometric (fura-2) monitoring of the cytosolic concentrations of Ca2+ ([Ca2+]i) and fluorochrome. In all of the cells an exposure to 100 500 μM PlnA induced a nearly instant permeabilization of the membrane, indicated by the following criteria: increased membrane conductance, membrane depolarization, increased [Ca2+]i, and diffusional loss of fluorochrome from the cytosol. When exposed to 100 μM PlnA, none of the inside-out patches from Vero cells were permeabilized. On the contrary, more than 80 % of the outsideout patches were permeabilized at 10 μM PlnA. We thus conclude that the permeabilizing effect of PlnA is not restricted to neither of the normal tested cell types nor the cancerous cells, and that PlnA differentiate between the inner and outer membrane leaflet.eng
dc.language.isonoben_US
dc.subjectantimikrobielle peptider nikrofluorometri bakteriosin kreft antibiotikaen_US
dc.titleDet antimikrobielle peptidet Plantaricin A permeabiliserer lever- og nyrecelleren_US
dc.typeMaster thesisen_US
dc.date.updated2009-03-31en_US
dc.creator.authorAndersland, Kristinen_US
dc.creator.authorJohansen, Guro Fiveen_US
dc.date.embargoenddate10000-01-01
dc.rights.termsDette dokumentet er ikke elektronisk tilgjengelig etter ønske fra forfatter. Tilgangskode/Access code Aen_US
dc.rights.termsforeveren_US
dc.subject.nsiVDP::473en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Andersland, Kristin&rft.au=Johansen, Guro Five&rft.title=Det antimikrobielle peptidet Plantaricin A permeabiliserer lever- og nyreceller&rft.inst=University of Oslo&rft.date=2009&rft.degree=Masteroppgaveen_US
dc.identifier.urnURN:NBN:no-21291en_US
dc.type.documentMasteroppgaveen_US
dc.identifier.duo88471en_US
dc.contributor.supervisorTrude M. Haug og Olav Sanden_US
dc.identifier.bibsys082929076en_US
dc.rights.accessrightsclosedaccessen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/11417/1/Oppgaven_Kristin_og_Guro14.pdf


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