The human HMGA2 gene is located at chromosomal band 12q14-15. In normal cells itencodes for the HMGA2 protein (109 amino acids). HMGA2 is a transcription factorthat binds to the AT rich sequences on DNA and thereby changing its conformation.The gene consists of five exons and between the third and the fourth exon, there is anintron with a size of 140Kb which may be broken in tumours. Such rearrangementshave been identified in benign neoplasms and several types of malignantmesenchymal tumours. Mesenchymal stem cells (MSCs) are multipotent stem cellscapable of differentiating into adipocytes among others.In this study we have induced adipogenic differentiation in the immortalized MSCline iMSC#3b. We found that theses cell lines are capable of differentiating intoadipocytic lineage but difficult to transfect, and changed to another MSC line,hMSCtert20. hMSC-Tert20-HMGA2 clone t4 cell line was used for overexpression ofHMGA2 during adipogenic differentiation and expression levels were measured byreal time RT-PCR. It turned out that forced expression of exogeneous HMGA2 led tofurther overexpression during differentiation in most of the measurements. The effectof overexpression of HMGA2 was investigated on one of the markers of adipogenicdifferentiation Peroxisome proliferator-activated receptor γ (PPARγ). Also PPARγexpression was enhanced during differentiation by overexpressing HMGA2. It wasalso possible to see a slight increase in its expression in cells not induced foradipogenesis.We have also attempted to confirm the HMGA2 and NF-kB interaction. We usedantibodies against HMGA2 and NF-kB-p65 to to investigate the expected interactionby immunoprecipitation. No interaction could be detected in the samples (HMGA2and NF-kB) investigated, but this could be due to low sensitivity.