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dc.date.accessioned2013-03-12T08:34:59Z
dc.date.available2013-03-12T08:34:59Z
dc.date.issued2011en_US
dc.date.submitted2011-07-26en_US
dc.identifier.citationHelm, Paul Johannes, Ottersen, Ole Petter, . Proposal of a new method to measure FRET quantitatively in living or fixed biomedical specimens on a laser microscope. Proceedings of SPIE, the International Society for Optical Engineeringen_US
dc.identifier.urihttp://hdl.handle.net/10852/11296
dc.description.abstractFörster Resonance Energy Transfer , abbreviated FRET , is a fluorescence phenomenon, which can be used to study and map co-localizations and dynamics of co-localizations at nanometer precision on a light microscope. FRET has been described as a spectroscopic ruler . The efficiency of the radiationless energy transfer from an excited chromophore, the donor , to another chromophore, the acceptor , the excitation energy of which approximately matches the energy to be released by the donor, is dependent on the sixth power of the mutual distance between the two molecules in space. We propose a new, non-destructive technique for measuring FRET quantitatively and at high spatial and temporal resolution on a laser scanning microscope: Two laser beams of wavelengths suitable for the mutually exclusive excitation of the donor and the acceptor, the donor beam and the acceptor beam , respectively, are intensity modulated by means of two electro optical modulators (EOM). The modulation patterns are rectangular at duty cycle ½. The modulation frequencies differ slightly. The acceptor beam is saturating the acceptor so that it cannot accept energy from the donor. The saturation is modulated in the same way as the acceptor beam. Since the donor beam also is modulated, though at a frequency slightly different from that of the acceptor beam, the intensity of the released donor fluorescence is modulated with the beat frequency of the frequencies of the two laser beam modulations and can be detected and interpreted in quantitative terms by means of a lock in amplifier. Copyright 2011 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.eng
dc.language.isoengen_US
dc.titleProposal of a new method to measure FRET quantitatively in living or fixed biomedical specimens on a laser microscopeen_US
dc.typeJournal articleen_US
dc.date.updated2011-08-01en_US
dc.creator.authorHelm, Paul Johannesen_US
dc.creator.authorOttersen, Ole Petteren_US
dc.subject.nsiVDP::473en_US
cristin.unitcode152000en_US
cristin.unitnameMolekylær biovitenskapen_US
dc.identifier.cristin829570en_US
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Proceedings of SPIE, the International Society for Optical Engineering&rft.volume=7903en_US
dc.identifier.jtitleProceedings of SPIE, the International Society for Optical Engineering
dc.identifier.volume7903
dc.identifier.startpage790331-1
dc.identifier.endpage790331-13
dc.identifier.doihttp://dx.doi.org/10.1117/12.874073
dc.identifier.urnURN:NBN:no-28509en_US
dc.type.documentTidsskriftartikkelen_US
dc.identifier.duo132863en_US
dc.type.peerreviewedPeer revieweden_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/11296/1/7903_107.pdf
dc.type.versionPublishedVersion


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